Catalog Number - 62308-25P

Hyper Similars C8™ Octyl is a traditional reversed-phase HPLC stationary phase developed for laboratories seeking a high-quality alternative to established C8 methods. As part of the Cogent™ Similars product line, it uses high-purity Type-B spherical silica with a bonded octyl (C8) ligand and endcapping to provide reliable chromatographic performance and excellent method reproducibility. Its surface characteristics are designed to closely match those of legacy Hypersil® and Spherisorb® C8 columns, making it particularly useful for method transfer and replacement applications.

62308-25P is a 250 mm x 4.6 mm ID analytical column packed with 3 µm, 150Å Hyper Similars C8 particles. The 250 mm length provides increased resolving power and higher theoretical plate counts than shorter columns, making it well suited for impurity profiling, complex sample separations, and methods requiring maximum chromatographic resolution. The 3 µm particle size delivers greater efficiency and sharper peak shapes than comparable 5 µm columns, although higher operating backpressure should be expected. The industry-standard 4.6 mm ID format typically operates optimally at approximately 1.0–2.0 mL/min on conventional HPLC systems.

This USP L7 column is an excellent alternative for methods developed on Thermo Fisher Hypersil™ C8, Spherisorb®, and similar octyl phases. The C8 chemistry is ideal for hydrophobic and neutral compounds analyzed under reversed-phase conditions and is particularly useful when less retention is desired than with a C18 column. Common applications include long-chain fatty acids, pharmaceutical compounds, environmental analyses, and method transfer projects requiring selectivity comparable to traditional C8 stationary phases.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L7 methods

• Comparable selectivity to Hypersil® and Spherisorb® C8 columns

• Increased resolution from 250 mm column length

• Higher efficiency with 3 µm particles

• Sharper peaks and improved separation performance

• Less retention than C18 columns

• End-capped octyl (C8) bonded phase

Typical Applications

• USP L7 methods

• Reversed-phase HPLC

• Pharmaceutical analysis

• Impurity profiling

• Method development

• Long-chain fatty acid analysis

• Environmental testing

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Hyper Similars C8 Octyl

• USP Classification: L7

• Particle Size: 3 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C8 (Octyl)

• Endcapped: Yes

• Pore Size: 150Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Length: 250 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Longer column length provides increased resolving power

• 3 µm particles provide higher efficiency and sharper peaks

• Higher backpressure than comparable 5 µm columns

• Compatible with conventional HPLC systems

• Provides less retention than comparable C18 stationary phases

• Excellent alternative for many USP L7 methods

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